Deletion of FNIP1 does not affect AMPK and mTORC1 signaling in adipocytes. (A and C) Representative Western blot analysis of iWAT (A) or BAT (C) lysates for male mice of the indicated genotype. (B and D) Quantification of Western blot results in A or C. n = 4–13 mice per group. (E) Representative Oil-Red-O staining of differentiated white adipocyte isolated from iWAT of male WT and FNIP1 KO mice at low magnification (top) and at high magnification (bottom). Scale bars: 10×, 100 μm; 20×, 50 μm. n = 3 independent experiments. (F) Gene expression (qRT-PCR) in primary adipocytes. n = 4 independent experiments. (G) Representative Western blot analysis performed with differentiated white adipocyte isolated from iWAT of male WT and FNIP1 KO mice. (H) Quantification of Western blot results in G. n = 3 independent experiments. (I) Representative Western blot analysis of p-S6 activation in WT and FNIP1 KO adipocytes. Cells were starved (50 min) or starved and re-stimulated with amino acids (AA) for 10 min, as indicated. Quantification of the p-S6/S6 signal ratios were presented below the corresponding bands. n = 3 independent experiments. Values represent mean ± SEM. P value was determined using two-tailed unpaired Student’s t-test. Data are representative of at least three independent experiments (A, C, E–G, and I). Source data are available for this figure: SourceData F3.