Loss of adipocyte FNIP1 leads to increased respiration and cold tolerance. (A) Body weight of indicated chow-fed 10-wk-old male mice. n = 3–4 mice per group. (B–D) Energy expenditure rates (VO2, VCO2, and heat production) of chow-fed 10-wk-old male mice after CL injection (0.5 mg/kg). n = 3–4 mice per group. (E) Rectal temperature recordings taken hourly during 4 h of cold exposure using a rectal digital probe in the fed state. n = 5–7 male mice per group. (F) Rectal temperature recordings taken hourly during 3 h of cold exposure using a rectal digital probe. Notably, male mice were fasted for 12 h before cold-exposure experiments. n = 7–8 mice per group. Values represent mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001. P value was determined using two-tailed unpaired Student’s t-test (A), or two-way ANOVA and Fisher’s LSD post-hoc test (B–F). Data are representative of three independent experiments (E).
