Impact of pneumococcal capsule on hepatic trapping. (A) Illustration of experimental detection of bacteria localization in CD1 mice after i.v. infection with 106 CFU of isogenic TH870 derivatives. (B) Bacteremia kinetics of WT or acapsular (Δcps) strain in the first 30 min of infection. n = 5. (C) Proportional distribution of WT and Δcps in the blood and organs at 5 min after infection. n = 3. (D) Bacterial load in the liver at 5 min. n = 3. (E) Bacteremia kinetics of HV (red) and LV (green) serotypes as in B. n = 5–6. (F) Proportional distribution of HV (red) and LV (blue) serotypes in the blood and organs at 5 min. n = 3. (G) Bacterial load in the liver at 5 min as in F. (H) Viable isogenic HV and LV pneumococci detected in the blood and five major organs of mice at 5 min after infection. n = 3. (I) Kinetics of viable HV (left) and LV (right) pneumococci in the liver of mice during the first 30 min of infection. n = 3–9. (J) Kinetics of viable pneumococci detected in the blood and organs in the first 30 min. The CFU values are presented as ratios of the corresponding inoculum sizes. n = 3–9. (K and L) Bacteremia kinetics (K) and proportional distribution (L) of serotype-8 and -14 pneumococci in SPX and SHM mice in the first 30 min of infection. n = 3–6. (M and N) Bacterial load (M) and proportional distribution (N) of serotype-8 and -14 pneumococci 12 h after i.t. instillation with 107 CFU. n = 3–6. Data were from one experiment (C, D, and F–H) or pooled from two independent experiments (B, E, and I–N). Unpaired t test (D), ordinary one-way ANOVA with Dunnett’s multiple comparisons test (G), ****, P < 0.0001.
