Altered odontoblastic and endothelial heterogeneity in Vegfr2 ^iΔEC mice. (A) Schematic diagram depicting the strategy for scRNA-seq analysis of dental pulp cells. (B and C) UMAP plot of cells from control and Vegfr2^iΔEC mice at P12. Clustering was performed based on expression of canonical cell-type markers for HCs (HC1, HC2, and HC3), odontoblasts (OB1 and OB2), myelinated Schwann cells (MScCs), non-myelinated Schwann cells (NMScC1 and NMSsC2), EC1s and EC2s, and mesenchymal stem cells (MSCs). (D) Cell counts (%) for each cluster shown in B. Red color indicates FC >2.5. (E) Gene ontologies for differentially expressed genes upregulated or downregulated in Vegfr2^iΔEC compared with control mice. Eight representative ontologies sorted from the top 25 ontologies are shown. (F) Heatmaps showing expression level of dentinogenesis genes extracted from the gene list GOBP_ODONTOGENESIS_OF_DENTIN_CONTAINING_TOOTH (M15056) or Hif-1 target genes extracted from the list SEMENZA_HIF1_TARGETS (M12299) in the website for gene set enrichment analysis (https://www.gsea-msigdb.org/gsea/msigdb/index.jsp).