Figure 4.
![POTCs overgrow by Vegfr1 deletion. (A) Protocol for 4OHT injection. (B) Bright-field view of mandibles. Vegfr1iΔEC mice had intact molar extrusion. (C–J) Section specimens at P12 and quantification (I, control [Cont] n = 3, Vegfr1iΔECn = 3; J, Cont n = 4, Vegfr1iΔECn = 4). Vegfr1iΔEC mice exhibited increased vessel area and filopodia extension (closed arrowheads). (K–N) Section specimens at P12. Vegfr1;Vegfr2iΔEC mice had fewer POTCs (closed arrowheads). Scale bars: 500 µm (B); 200 µm (C, D, K, and L); 50 µm (E–H, M, and N). Data are presented as means ± SD. Comparisons between two groups were evaluated using a two-sided Student’s t test.](https://cdn.rupress.org/rup/content_public/journal/jem/219/4/10.1084_jem.20211789/5/jem_20211789_fig4.png?Expires=1772019904&Signature=LgI57qm7zOWI2qX0XS2k~dSxoUR9XI2vXWsfI7Fou1Zc9qHLejsj5SsXkbj2~hnE09RStFKVINfouQ5i~oSjYl5zoR8VhDMU3oo~qE73A6RyYpyJfbgQFLtXQ~T1KFklJoR0gzzcatEegWOLQ81Uq7YafCauhdQp~KzsZLQ8IENuUkmu-cbuPAfT8crY6QMCUa9xyyPKEzaz0T7lLhg49IiXrHqsTW589RFk6JCGAcZZRsCBHpdWyKbMqgtUGx0qR-Z-osfPrDo-evl2lI15XMJj~2ihP0hiL0j8BW5RrqVU1Rj04SvO~sAFIoh4Jm8KgtfRPHDP2igYno-bIMgsfA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
POTCs overgrow by Vegfr1 deletion. (A) Protocol for 4OHT injection. (B) Bright-field view of mandibles. Vegfr1iΔEC mice had intact molar extrusion. (C–J) Section specimens at P12 and quantification (I, control [Cont] n = 3, Vegfr1iΔECn = 3; J, Cont n = 4, Vegfr1iΔECn = 4). Vegfr1iΔEC mice exhibited increased vessel area and filopodia extension (closed arrowheads). (K–N) Section specimens at P12. Vegfr1;Vegfr2iΔEC mice had fewer POTCs (closed arrowheads). Scale bars: 500 µm (B); 200 µm (C, D, K, and L); 50 µm (E–H, M, and N). Data are presented as means ± SD. Comparisons between two groups were evaluated using a two-sided Student’s t test.
