DSTYK inhibition affects mitochondrial morphology and functionality . (A) FACs analysis of mitochondrial mass (Mitotracker) and membrane potential (TMRM) of parental or KO LLC and H2009 cells in stress conditions. Three experimental replicates were performed for each experimental condition. Analysis by t test rendered TMRM/Mitotracker ratio MFI, P < 0.0001. (B) Detailed confocal microscopy images of mitochondrial ring-shape in parental or KO H2009 cells under stress conditions. Scale bar: 20 µm. (C) Confocal microscopy images of parental or KO H2009 cells under basal or stress conditions 16 h after treatment. Scale bar: 20 µm. ***, P < 0.001. Panels show representative results from at least two independent experiments performed.