Figure 3. DSTYK role in oxidative stress... | Rockefeller University Press

Figure 3.

$DSTYK role in oxidative stress in lung cancer cells. (A) Cropped images from the Western blot analysis of KEAP1/NRF2 pathway proteins in LLC and H2009 parental and DSTYK-KO cells in cytoplasmic/nuclear fractions. Western blots show representative results from three independent experiments. (B) ROS measurements in LLC and H2009 parental and DSTYK-KO cells in basal conditions. Three experimental replicates were performed for each experimental condition. Experimental groups were compared by t test: LLC, P = 0.0031; and H2009, P = 0.0011. (C) Seahorse extracellular flux analysis shows dynamic representation of oxygen consumption rate (OCR) in LLC and H2009 cells under stress conditions. Three experimental replicates were performed for each experimental condition. (D) Comparison of different phases of mitochondrial respiration. Parental or KO LCC and H2009 cells share alteration of maximal respiratory and spare respiratory capacity in stress conditions. Experimental groups were compered by t test. LLC relative maximal respiration, P = 0.03; relative spare capacity, P = 0.01. H2009 relative ATP production, P = 0.002; relative proton leak, P = 0.04; relative maximal respiration, P = 0.0008; relative spare capacity, P < 0.0001; relative non-mitochondrial oxygen consumption, P = 0.03. *, P < 0.05; **, P < 0.01; ***, P < 0.001. All images are representative results out of three experimental replicates. Source data are available for this figure: SourceData F3.$

DSTYK role in oxidative stress in lung cancer cells. (A) Cropped images from the Western blot analysis of KEAP1/NRF2 pathway proteins in LLC and H2009 parental and DSTYK-KO cells in cytoplasmic/nuclear fractions. Western blots show representative results from three independent experiments. (B) ROS measurements in LLC and H2009 parental and DSTYK-KO cells in basal conditions. Three experimental replicates were performed for each experimental condition. Experimental groups were compared by t test: LLC, P = 0.0031; and H2009, P = 0.0011. (C) Seahorse extracellular flux analysis shows dynamic representation of oxygen consumption rate (OCR) in LLC and H2009 cells under stress conditions. Three experimental replicates were performed for each experimental condition. (D) Comparison of different phases of mitochondrial respiration. Parental or KO LCC and H2009 cells share alteration of maximal respiratory and spare respiratory capacity in stress conditions. Experimental groups were compered by t test. LLC relative maximal respiration, P = 0.03; relative spare capacity, P = 0.01. H2009 relative ATP production, P = 0.002; relative proton leak, P = 0.04; relative maximal respiration, P = 0.0008; relative spare capacity, P < 0.0001; relative non-mitochondrial oxygen consumption, P = 0.03. *, P < 0.05; **, P < 0.01; ***, P < 0.001. All images are representative results out of three experimental replicates. Source data are available for this figure: SourceData F3.

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