Distribution of maternally transferred VIP-derived mAbs and directly administered mAbs in pups. Magnetic bead-based assays were used to detect hIgG transfer and biodistribution. (A) Detection of in vivo expressed huIgG in the serum (diluted 1–300) of 2-d-old progeny of AAV-administered dams, each column represents an individual AAV-transduced dam, and each symbol within the column a mouse pup, the line represents the median value. CH42 (dam n = 1, pup n = 4); Ctrl IgG (dam n = 3, pup n = 9); CH43 (dam n = 2, pup n = 6); E317 (dam n = 2, pup n = 6). (B) Biodistribution of huIgG of pups 48 h after intraperitoneal administration of mAbs UB-621 (pup n = 2), CH42 (pup n = 2), HSV8 (pup n = 2). Each symbol represents a mouse pup. Signal is reported as the fold increase in huIgG in treated pups relative to untreated controls. Serum (SRM), stomach (STM), lung (LNG), liver (LVR), heart (HRT), kidney (KDY), spleen (SPL), trigeminal ganglia (TG), brain (BRN).