Administration of CH42 reduces viral dissemination. Pregnant dams or 2-d-old pups received intraperitoneal injections of CH42 or IgG control (ctrl) antibody before or after intranasal viral challenge with a luciferase-expressing reporter HSV-1 (1 × 105 PFU). Pups were imaged on DPI 2, then imaged until DPI 8. Representative images follow the same two pups sequentially. (A) Bioluminescence imaging of pups following CH42 (dam n = 2, two litters, pup n = 10) or IgG control (dam n = 2, two litters, pup n = 12) administration to pregnant dams 5 d before infection. (B) Bioluminescence imaging of pups following i.p. mAb administration of CH42 (dam n = 1, one litter, pup n = 6) or IgG control (dam n = 1, one litter, pup n = 5) and immediate subsequent viral challenge. (C) Bioluminescence imaging of pups following i.p. mAb administration of CH42 (dam n = 2, two litters pup n = 4–6) or IgG control (dam n = 2, pup n = 4–6) 1 d after infection. (D) The quantification of the virally derived bioluminescence in A–C in the top, middle, and bottom panels, respectively. Each dot represents an animal, the solid line is the median, and the dotted lines are the quartiles. Statistical significance was determined by two-way ANOVA, and Šídák’s method for multiple comparisons (**, P < 0.01; ***, P < 0.001; ****, P < 0.0001). DPI, days post infection.
