T1B, T2B, and B1 cells occupy distinct regulatory states. (A) GRN-based clustering color coded by B cell state and projected on a t-distributed stochastic neighbor embedding (t-SNE) map reveals that T1B, T2B, and B1 have distinct regulatory network structures. (B) GRN density projected onto SCENIC t-SNE map. (C) Rank-ordered regulon specificity scores for transcriptionally defined B cell states. (D–F) t-SNE map plotting gene expression (yellow), binarized regulon activity (active, blue; inactive, gray), and kernel density line area under the curve (AUC) histogram plotting inferred transcription factor (TF) activation. Examples of differentially active GRNs in T1B (D), T2B (E), and B1 (F) states.