SARS-CoV-2 viral load in apical wash, and ISG expression in organoids following pretreatment with BX795 in sequential RV, SARS-CoV-2 infection. Related to Fig 6, A–D. (A–C) Organoid cultures were pretreated with or without BX795 for 18 h, mock infected or infected with HRV-01A, incubated for 3 d, and then infected with SARS-CoV-2, MOI 0.5 (from the experiment shown in Fig 6, A–D). SARS-CoV-2 viral RNA level relative to the limit of detection in RNA isolated from the apical wash collected 72 h after SARS-CoV-2 infection, with and without BX795 and/or RV pretreatment. ISG mRNA levels relative to levels in mock-treated cultures. Bars show mean, and symbols show individual replicates. Bars show mean and SEM of five or six replicates per condition. Symbols indicate significant difference by Mann–Whitney test (***, P = 0.0043; ^##, P = 0.0079; **, P = 0.0095; *, P = 0.0159). Data are representative of two independent experiments each with at four to six biological replicates per condition.