Figure S3.
Expression level of full-length ACE2 and the truncated variant dACE2 in mock-treated airway epithelial organoids following RV infection (HRV-01A). Related to Fig 5. (A) Organoids were mock infected or infected with RV. Some RV-infected cultures were pretreated for 18 h with 6 μM BX795 before infection with RV. (B) RT-qPCR was performed to quantify transcripts for full-length ACE2 (blue circles) or dACE2 (white triangles). Plot shows compiled data from three independent experiments, each with three to five biological replicates per condition. Individual values are shown (10–13 per condition). Numbers indicate P values of the Mann–Whitney test for significant differences between conditions. The primers used for full-length ACE2 target the junction of exons 8 and 9 are unique to the full-length ACE2, and the primers used for dACE2 target the junction of exons 1 and 2 are unique to dACE2, as previously described by Onabajo et al., 2020.

Expression level of full-length ACE2 and the truncated variant dACE2 in mock-treated airway epithelial organoids following RV infection (HRV-01A). Related to Fig 5. (A) Organoids were mock infected or infected with RV. Some RV-infected cultures were pretreated for 18 h with 6 μM BX795 before infection with RV. (B) RT-qPCR was performed to quantify transcripts for full-length ACE2 (blue circles) or dACE2 (white triangles). Plot shows compiled data from three independent experiments, each with three to five biological replicates per condition. Individual values are shown (10–13 per condition). Numbers indicate P values of the Mann–Whitney test for significant differences between conditions. The primers used for full-length ACE2 target the junction of exons 8 and 9 are unique to the full-length ACE2, and the primers used for dACE2 target the junction of exons 1 and 2 are unique to dACE2, as previously described by Onabajo et al., 2020.

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