Confirmation of the LEC-specific loss of VCAM-1 in Prox1-Cre^ERT2 Vcam1^fl/fl mice. (A) LEC-specific deletion of VCAM-1 was induced in Prox1-Cre^ERT2 Vcam1^fl/fl mice by i.p. administration of tamoxifen. (B and C) Deletion of VCAM-1 was confirmed by analyzing VCAM-1 expression in IFNγ- and TNFα-stimulated primary LECs isolated from tail skin of tamoxifen-treated Cre⁺ (Prox1-Cre^ERT2 Vcam1^fl/fl) or Cre⁻ control mice (WT or Vcam1^fl/fl). Representative FACS plots with respective percentages of VCAM-1⁺ cells are shown in B. Isotype control is shown as a gray shaded histogram. Quantification of percentage of VCAM-1⁺ cells is presented in C. Data from three experiments are shown. (D and E) FACS-based analysis of VCAM-1 expression in endothelial cells present in TPA-inflamed ear skin of Cre⁺ or Cre⁻ control mice. Shaded histogram: VCAM-1. Empty histogram: Isotype control. (D) Representative FACS plots. (E) Summary of the delta median fluorescent intensities (ΔMFI: specific-isotype staining) from all experiments performed (n = 4 mice per genotype). Measurements from the same mouse are connected by a line. (G) Summary of the ΔMFIs measured per experiment between colLECs and capLECs. (C and F) Unpaired Student’s t test. (E) Paired one-way ANOVA with the Geisser–Greenhouse correction. *, P < 0.05; **, P < 0.01; and ****, P < 0.0001. cap, capillaries; col, collectors; Max, maximum.