Collector-specific expression of adhesion molecules and chemokines in inflamed lymphatic collectors. (A) Heatmaps of adhesion molecule and chemokine genes involved in leukocyte migration. (B) Reads per kilobase per million mapped reads (RPKM) plots of Cx3cl1, Cxcl12, and Vcam1 in steady-state (CTR) and INF BECs, colLECs, and capLECs. Box shows median, 25%, and 75% percentile; whiskers show minimum and maximum. Representative whole-mount images showing differential expression of (C) CX3CL1, (D) CXCL12, and (E) VCAM-1 in lymphatic vessels in CTR and INF murine ear skin (n = 3 mice per condition). In the case of VCAM-1 (E), the corresponding isotype staining is shown. (C–E) White arrows indicate collectors, yellow arrows capillaries. Scale bars, 50 µm. (F–I) Analysis of VCAM-1 expression in single-cell suspensions generated from CTR and INF murine ear skin. (F) Representative FACS plots showing VCAM-1 expression in capLECs (CD45⁻CD31⁺podo⁺LYVE-1⁺), colLECs (CD45⁻CD31⁺podo⁺LYVE-1⁻), and BECs (CD45⁻CD31⁺podo⁻LYVE-1⁻) under CTR and INF conditions. Shaded histogram, VCAM-1; empty histogram, isotype control. (G) Summary of the delta median fluorescent intensities (ΔMFI: specific-isotype staining) from all experiments performed under CTR (n = 5 mice) or INF (n = 7 mice) conditions. Measurements from the same sample are connected by a line. (H) Summary of MFIs measured for colLECs in CTR or INF samples. (I) Summary of the ΔMFIs measured per experiment between colLECs and capLECs. (G–I) Paired one-way ANOVA with Geisser–Greenhouse correction (G) and unpaired Student’s t test (H and I); *, P < 0.05; **, P < 0.01. cap, capillaries; col, collectors; Max, maximum; podo, podoplanin.