RNA sequencing of LECs derived from lymphatic capillaries and collectors and BECs isolated from murine skin. (A) Representative whole-mount images of steady-state mouse ear skin showing dermal blood vessels (CD31⁺podo⁻LYVE-1⁻), lymphatic collectors (CD31⁺podo⁺LYVE-1⁻; white arrows) and lymphatic capillaries (CD31⁺podo⁺LYVE-1⁺; yellow arrows). Scale bar, 50 µm. (B) Principal component analysis of capLECs, colLECs, and BECs from CTR and INF conditions (n = 5 experimental replicates). (C) Reads per kilobase per million mapped reads (RPKM) plots of vascular marker genes. Box shows median, 25%, and 75% percentile; whiskers show minimum and maximum. (D) Volcano plot of capLECs versus colLECs in steady-state (CTR) conditions (0.5-fold change; P value, 0.05). (E–G) FACS was performed on CTR skin single-cell suspensions following the gating scheme in Fig. S2 A. Analysis of MRC1 (E), ESAM (F), and ITGB3 (G). Representative FACS plots and ΔMFI of three independent experiments are shown. (H and I) Identification of specific differentially regulated pathways in CTR versus INF capLECs (H) and colLECs (I). MetaCore Process network 1.5-fold change; P value, 0.05; deregulated pathways. Max, maximum; MFI, median fluorescent intensity.