Bioinformatic analysis of HSPCs homed into 5-FU–treated BM to determine activated signals. (A–F) Bioinformatic analyses of RNA-seq data were conducted. Sequencing data shown here are overlapped with data shown in Fig. 1 C. (A) The top 10 enriched biological functional categories between two groups, according to the Ingenuity Knowledge Base, are listed. The x axis shows the significance, which is the value of –log(P value). (B) Color-coded heat map analyses regarding “cell cycle” and “cell death and survival” among enriched biological functional categories. (C) Subcategories in the highlighted heat map of “cell death and survival” category within B. (D and E) Upstream transcriptional regulators that were significantly activated (z-score > 2; D) or inhibited (z-score < −2; E) in HSPCs transplanted into 5-FU–treated mice compared with those into control mice. (F) Upstream cytokine/growth factors that were significantly activated (z-score > 2) in HSPCs transplanted into 5-FU–treated mice compared with those into control mice. (G) Differentially expressed genes from RNA-seq in this study (bioset 1) were compared with those in another study (bioset 2), in which gene expression of GM-CSF–stimulated versus unstimulated dendritic cells in vitro was analyzed (Min et al., 2012), using the BaseSpace Correlation Engine (Illumina). A strong positive correlation was observed between the two biosets. The Venn diagram shows the number of differentially expressed genes that are overlapped in the two biosets. The bar graph shows the significance levels of overlapped or unique genes by –log(P value). GSE, Gene Expression Omnibus Series.