Figure 2.
CGRP mediates the protective effects of nociceptive pathway. (A) Diagram of experimental design. (B–E) Number of adherent leukocytes (B), rolling leukocytes (C), and WBC-RBC heterotypic interactions (D) and blood flow rate (E) in in the postcapillary collecting venues of cremaster muscle in vehicle-treated SCD mice (n = 52 venules from five mice) and CRPP-treated SCD mice (n = 43 venules from five mice) analyzed by intravital microscopy. (F) Survival time of vehicle-treated SCD mice (n = 5) and CGRP-treated SCD mice (n = 5) in TNFα-induced acute vaso-occlusion. (G and H) Percentage of aged neutrophils (G) and absolute number of aged neutrophils (H) in peripheral blood of vehicle-treated SCD mice (n = 5) and CGRP-treated SCD mice (n = 5). (I) Diagram of experimental design. (J–L) Number of adherent leukocytes (J) and rolling leukocytes (K) and blood flow rate (L) in the postcapillary collecting venues of cremaster muscle in SCD →Ramp1+/+ mice (n = 30 venules from eight mice) and SCD → Ramp1−/− mice (n = 37 venules from six mice) analyzed by intravital microscopy. (M) Survival time of SCD →Ramp1+/+ mice (n = 8 mice) and SCD →Ramp1−/− mice (n = 6 mice) in TNFα-induced acute vaso-occlusion. Error bars, mean ± SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Data represent at least two independent experiments analyzed using unpaired Student’st test in B–E, G, H, and J–L and Mantel–Cox test (log-rank) for the survival analysis in F and M. ns, not significant.

CGRP mediates the protective effects of nociceptive pathway. (A) Diagram of experimental design. (B–E) Number of adherent leukocytes (B), rolling leukocytes (C), and WBC-RBC heterotypic interactions (D) and blood flow rate (E) in in the postcapillary collecting venues of cremaster muscle in vehicle-treated SCD mice (n = 52 venules from five mice) and CRPP-treated SCD mice (n = 43 venules from five mice) analyzed by intravital microscopy. (F) Survival time of vehicle-treated SCD mice (n = 5) and CGRP-treated SCD mice (n = 5) in TNFα-induced acute vaso-occlusion. (G and H) Percentage of aged neutrophils (G) and absolute number of aged neutrophils (H) in peripheral blood of vehicle-treated SCD mice (n = 5) and CGRP-treated SCD mice (n = 5). (I) Diagram of experimental design. (J–L) Number of adherent leukocytes (J) and rolling leukocytes (K) and blood flow rate (L) in the postcapillary collecting venues of cremaster muscle in SCD →Ramp1+/+ mice (n = 30 venules from eight mice) and SCD → Ramp1−/− mice (n = 37 venules from six mice) analyzed by intravital microscopy. (M) Survival time of SCD →Ramp1+/+ mice (n = 8 mice) and SCD →Ramp1−/− mice (n = 6 mice) in TNFα-induced acute vaso-occlusion. Error bars, mean ± SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Data represent at least two independent experiments analyzed using unpaired Student’st test in B–E, G, H, and J–L and Mantel–Cox test (log-rank) for the survival analysis in F and M. ns, not significant.

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