Tamoxifen induces deletion of CaMKIIδ in endothelial cells in vivo. The inducible VE-Cadherin CaMKIIδ^fl/fl mice exhibit selective deletion of CaMKIIδ in endothelial cells after induction of the Cre recombinase with tamoxifen. (A) Immunofluorescence of the whole mount of the control aorta demonstrates CaMKIIδ expression in endothelial cells. (B) Immunofluorescence of whole mount of the aorta after tamoxifen injections shows that expression of CaMKIIδ is specifically deleted in endothelial cells. Residual staining that appears as streaks in the tamoxifen-treated, CaMKIIδ-stained sample is an artifact created by folds during the tissue mounting process and not residual expression. Furthermore, deletion of CaMKIIδ does not have any effect on endothelial cell morphology or PECAM expression. At least three fields from three independent experiments were evaluated, and representative images are shown. Scale bar is 10 µm.