Tamoxifen induces endothelial cell CaMKIIN expression in vivo. The inducible VE-Cadherin CaMKIIN mice expressed a floxed eGFP sequence upstream of a stop codon followed by an HA-tagged CaMKII inhibitor peptide, CaMKIIN (HA-tagged CaMKIIN). Once Cre recombinase activity was induced with tamoxifen, the floxed GFP/stop codon sequence was excised, allowing expression of CaMKIIN selectively in endothelial cells. (A) Immunofluorescence of whole mount of the control aorta shows absence of HA-tagged CaMKIIN staining in control endothelial cells. (B) Immunofluorescence of whole mount of the aorta of mice injected with tamoxifen shows that expression of HA-tagged CaMKIIN is indeed specifically induced in endothelial cells. Furthermore, CaMKIIN expression does not have any effect on endothelial cell morphology or PECAM expression. At least three fields from three independent experiments were evaluated, and representative images are shown. Scale bar is 10 µm.