Figure 5.
Dominant negative CaMKIIδ markedly reduces monocyte TEM. (A) iHUVECs were transduced with dominant negative CaMKIIδ. 2 d after transduction, cells were lysed and their protein content resolved using SDS-PAGE and probed with Western blotting. Control (Cont) is shown for reference. GAPDH is shown as the loading control. (B) iHUVECs were fixed, permeabilized, stained for HA and VE-cadherin, and visualized using confocal immunofluorescence microscopy. Scale bar is 10 µm. (C) Monocyte transmigration across iHUVECs grown on collagen gels and transduced with dominant negative (DN) CaMKIIδ is shown. The mouse anti-human PECAM antibody hec7 was included as a control. Data represent the average and standard deviation of three independent experiments. Each experiment comprises at least three samples with >50 leukocytes scored per sample for each condition. ** denotes P value < 0.01 with Student’s t test. MW, molecular weight.

Dominant negative CaMKIIδ markedly reduces monocyte TEM. (A) iHUVECs were transduced with dominant negative CaMKIIδ. 2 d after transduction, cells were lysed and their protein content resolved using SDS-PAGE and probed with Western blotting. Control (Cont) is shown for reference. GAPDH is shown as the loading control. (B) iHUVECs were fixed, permeabilized, stained for HA and VE-cadherin, and visualized using confocal immunofluorescence microscopy. Scale bar is 10 µm. (C) Monocyte transmigration across iHUVECs grown on collagen gels and transduced with dominant negative (DN) CaMKIIδ is shown. The mouse anti-human PECAM antibody hec7 was included as a control. Data represent the average and standard deviation of three independent experiments. Each experiment comprises at least three samples with >50 leukocytes scored per sample for each condition. ** denotes P value < 0.01 with Student’s t test. MW, molecular weight.

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