Deficiency in LYVE1^hi macrophages delays intraperitoneal expansion of ovarian cancer in an omentum-independent manner. (A) Quantification of bioluminescence signals at different time points after tumor implantation (Csf1r^fl/fl mice, n = 12; Lyve1^ΔCsf1r mice, n = 13; mean ± SEM). (B) Bioluminescence images of tumor-bearing mice at 6 wk after inoculation. (C) Quantification of bioluminescence signal at different time points after inoculation in omentectomized (OMX) mice (Csf1r^fl/fl mice, n = 11; Lyve1^ΔCsf1r mice, n = 11; mean ± SEM). (D) Quantification of bioluminescence signal at different time points after inoculation of the omentum-primed ID8-A12 cells in OMX mice (Csf1r^fl/fl mice, n = 10; Lyve1^ΔCsf1r mice, n = 12; mean ± SEM). (E) Quantification of bioluminescence signal of ascites and mesenteries 4 wk after inoculation of the omentum-primed ID8-A12 cells in OMX mice (mean ± SEM). Unpaired Student’s t test: *, P < 0.05; ***, P < 0.001. Statistical analysis was performed using one-way ANOVA (A, C, and D) and Student’s t test (E).