Quantification of tissue-resident macrophages of Lyve1^Cre:Csf1r^fl/fl and control mice. (A) Gating strategy of two peritoneal macrophage subsets and their quantification in Lyve1^Cre:Csf1r^fl/fl mice and control mice. FSC, forward scatter; SSC, side scatter. (B) Gating strategy of splenic red pulp macrophages and their quantification in Lyve1^Cre:Csf1r^fl/fl mice and control mice. (C) Gating strategy of alveolar macrophages and their quantification of in Lyve1^Cre:Csf1r^fl/fl mice and control mice. (D) Gating strategy and percentage of microglia in CD45⁺ brain leukocytes of Lyve1^Cre:Csf1r^fl/fl mice and control mice. (E) Representative histogram of CSF1R expression in ICAM2⁺ and MHC II⁺ peritoneal macrophage subsets analyzed in A. In A–E, data were pooled from two independent experiments (n = 5–8 mice). Statistical analysis was performed by unpaired Student’s t test.