Characterization of membrane-associated macrophages. (A) Two-photon images of liver capsule from Lyz2^cre:R26^LSL-tdTomato:CD11c^EYFP mice. Scale bar, 70 µm. (B) Two-photon images of mesenteric membrane from Lyz2^cre:R26^Tomao:CD11c^EYFP mice. Scale bar, 40 µm. (C) Representative flow cytometric analysis of gut mesentery in CD11c^EFYP mice. CD11c^EYFP and CD11b gating of CD45⁺ MHCII^lo-to-hi mesenteric cells (left). Overlay of CD11b⁺ EYFP⁻ and CD11b⁺ EYFP⁺ cells (right; n = 6). (D) Whole-mount confocal images of Csf1r^CreER:R26^Tomato mice with CD206 and ICAM2 staining. Imaging data are representative of at least two independent experiments. Scale bar, 40 µm. (E) Flow cytometric analysis for ICAM2, CD206, MHC II, and CD226 expression in F4/80^hi LPMs, F4/80^lo small peritoneal macrophages (SPM) and F4/80^hi mesenteric macrophages. Data are representative of at least two independent experiments. (F) Mean fluorescent intensity (MFI) values of ICAM2, CD206, MHC II, and CD226, which are normalized by isotype controls. Data are pooled from at least two independent experiments (n = 3–6 mice). Statistical analysis was performed by one-way ANOVA and Tukey’s multiple-comparison test: **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.