Identification of genes correlated with loss of membrane budding in the FL and ABM. (Ai) Summary of up-regulated and down-regulated DE genes in comparisons of E13.5 FL Nfe2^+/+ versus Nfe2^−/− Mks, and ABM Nfe2^+/+ versus Nfe2^−/− Mks. All DE genes are listed in Data S1 and Data S2. (Aii) Scatter plot of Nfe2^+/+ versus Nfe2^−/− Mk gene expression log2 fold change from E13.5 FL and ABM sc RNA-seq. Highlighted are genes identified as significantly up-regulated (red) or down-regulated (blue); commonly up-regulated (yellow) or down-regulated (purple) at both developmental stages; and NFE2 direct target genes that are up-regulated (cyan) and down-regulated (green) in FL culture–derived Nfe2^−/− Mks (Fujita et al., 2013). (B) Heatmap of all common significantly DE genes. Rows were hierarchically clustered using 1 − Person correlation. (C) Complete list of enriched GO biological process terms for commonly up-regulated genes (i) and selected enriched GO biological process terms for commonly down-regulated genes (ii). See Table S5 for a complete list of enriched GO terms and Materials and methods for details of statistical analysis.