Figure 7.
ProPlt formation can precede exit of Mks from the BM.(A) Cumulative scores of observed outcomes of proPlt-forming BM Mks, derived from low-time-resolution 4D data over 4–5 h (o = 20; n = 3). (B) Example of a 3D intravital time series captured at 15 frames/s at a single z-position, showing the emigration of a polyploid Mk during proPlt formation. (Bi) 3D z-projection highlighting an Mk of interest (black arrow) before time-lapse imaging. (Bii) Snap shots from time series. Red arrow, leading edge of primary proPlt extension; pink arrows, nucleated region of proPlt-forming Mk leaving the BM space (nuclei as distinguished by the regional absence of membrane-bound GFP); cyan arrow, secondary proPlt extension formed after the disappearance of the nucleus. Pf4cre:mTmG non-Mk (red) and Mk (green); second harmonic signal generation, bone (gray). Scale bar, 50 µm.

ProPlt formation can precede exit of Mks from the BM. (A) Cumulative scores of observed outcomes of proPlt-forming BM Mks, derived from low-time-resolution 4D data over 4–5 h (o = 20; n = 3). (B) Example of a 3D intravital time series captured at 15 frames/s at a single z-position, showing the emigration of a polyploid Mk during proPlt formation. (Bi) 3D z-projection highlighting an Mk of interest (black arrow) before time-lapse imaging. (Bii) Snap shots from time series. Red arrow, leading edge of primary proPlt extension; pink arrows, nucleated region of proPlt-forming Mk leaving the BM space (nuclei as distinguished by the regional absence of membrane-bound GFP); cyan arrow, secondary proPlt extension formed after the disappearance of the nucleus. Pf4cre:mTmG non-Mk (red) and Mk (green); second harmonic signal generation, bone (gray). Scale bar, 50 µm.

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