Cellularity and Mk abundance in major Mk-containing organs. (A) Total cellularity of all major BM-containing bones, spleen and lungs (n = 3 mice). **, P = 0.008; *, P = 0.04, one-way ANOVA with Tukey’s multiple comparisons test. (B) Representative examples of the gating strategy used to identify mature Mks by flow cytometry in cell suspensions of femoral BM, spleen and lungs. Plots were derived from the exclusion of dead cells (7AAD⁺) and RBCs (TER119⁺); n = 3 mice. (C) Total number of circulating Plts in adult C57BL/6 mice (n = 48).