Figure S1.
Validation of in situ ploidy quantification in the E13.5 FL from high-volume 3D imaging data.(A) Representative examples of non-Mk (CD41−) mitotic figures identified using DAPI staining (i) and the derived surface objects (blue) used as 4N references to normalize the DNA content of Mks in situ (ii). Scale bars, 5 µm. (B) Nuclei within Mk surface objects (gray) pseudocolored (spectrum) according to DAPI intensity sum. Scale bar, 30 µm. (C) Mk ploidy profiles derived from the gold-standard flow cytometry assay (i) and from 3D images (ii). (D) Other than a greater frequency of 8N Mks classified using the imaging method, the two ploidy analysis techniques were highly comparable (n = 3 independent experiments). P = 0.008, one-way ANOVA.

Validation of in situ ploidy quantification in the E13.5 FL from high-volume 3D imaging data. (A) Representative examples of non-Mk (CD41−) mitotic figures identified using DAPI staining (i) and the derived surface objects (blue) used as 4N references to normalize the DNA content of Mks in situ (ii). Scale bars, 5 µm. (B) Nuclei within Mk surface objects (gray) pseudocolored (spectrum) according to DAPI intensity sum. Scale bar, 30 µm. (C) Mk ploidy profiles derived from the gold-standard flow cytometry assay (i) and from 3D images (ii). (D) Other than a greater frequency of 8N Mks classified using the imaging method, the two ploidy analysis techniques were highly comparable (n = 3 independent experiments). P = 0.008, one-way ANOVA.

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