Figure 1.
Identification of Plt-sized membrane buds.(A) Representative example of an in vitro proPlt from a E10.5 yolk sac Mk. Periodic swellings (yellow) interspersed by fine shafts (blue); n = 15 independent experiments. Scale bar, 20 µm. (B) Confocal 3D z-projections and optical sections from whole-mount yolk sac showing proPlt-like extensions (i, red arrows) and membrane buds (ii, yellow arrows). White arrow, free Plt; n = 15 independent experiments. Scale bars, 10 µm. (C) In vivo frequency of Plt-forming morphologies of Mks in the E10.5 yolk sac; n = 7 independent mice. P < 0.0001, unpaired two-tailed t test. (D) In vitro frequency of Plt-forming morphologies of E10.5 Mks after 3 d of culture (n = 15 independent experiments). P < 0.000001, unpaired two-tailed t test. (E and F) Membrane buds (arrows) associated with polyploid cells in E13.5 FL observed using scanning (E) and transmission (F) EM; n = 3 independent experiments.

Identification of Plt-sized membrane buds. (A) Representative example of an in vitro proPlt from a E10.5 yolk sac Mk. Periodic swellings (yellow) interspersed by fine shafts (blue); n = 15 independent experiments. Scale bar, 20 µm. (B) Confocal 3D z-projections and optical sections from whole-mount yolk sac showing proPlt-like extensions (i, red arrows) and membrane buds (ii, yellow arrows). White arrow, free Plt; n = 15 independent experiments. Scale bars, 10 µm. (C) In vivo frequency of Plt-forming morphologies of Mks in the E10.5 yolk sac; n = 7 independent mice. P < 0.0001, unpaired two-tailed t test. (D) In vitro frequency of Plt-forming morphologies of E10.5 Mks after 3 d of culture (n = 15 independent experiments). P < 0.000001, unpaired two-tailed t test. (E and F) Membrane buds (arrows) associated with polyploid cells in E13.5 FL observed using scanning (E) and transmission (F) EM; n = 3 independent experiments.

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