PR8 infection recruits LCMV-specific cells to the lung. (A) Staggered and contemporaneous (Contemp.) infection strategy. SAC, sacrifice. (B) LCMV alone (black circles) indicate i.v.^neg P14 cells in the lung 40–60 d after i.p. LCMV. After the staggered infection modality, 30 d after LCMV infection, mice received OT-I cells and PR8-ova i.n. 12 d later. OT-I (red) and P14 (gray) cells were enumerated in lung. Contemporaneous infection similarly recruited abundant LCMV-specific P14 cells into the lung early after infection (day 12); however, their maintenance was not sustained by 42 d after infection. Data representative of four mice per group from one of two independent experiments. Spc, specific.