Figure 1. A substantive subset of... | Rockefeller University Press

Figure 1.

$A substantive subset of influenza-specific memory CD8+ T cells isolated from the medLN express residency markers. (A) Mice received Thy1.1 P14 cells 1 d before i.n. infection with PR8-gp33. 85 d later, Thy1.1 expression was measured on P14 cells isolated from medLN by flow cytometry. (B) Ratio of Thy1.1bright to Thy1.1dim P14 cells isolated from the indicated tissues (plots were gated on the i.v.neg fraction of Thy1.1+ CD8+ T cells). Data displayed on a log2 scale, and numbers above bars represent ratio of Thy1.1bright to Thy1.1dim. (C and D) Phenotype of P14 cells described in A. Shaded quadrants highlight TRM phenotypes. (E) Ratio of Thy1.1+ P14 CD8+ T cells isolated from medLN versus lung nondraining SLO (40–60 d after i.n. influenza infection). Frequency of medLN P14 cells among total medLN CD8+ T cells set at 1. iLN, inguinal LN; cLN, cervical LN; mesLN, mesenteric LN. Scale is log2. (F) Histological comparison of P14 cell abundance and Thy1.1 staining intensity within the medLN and iLN 60 d after influenza infection. Data are represented as mean ± SEM. ***, P < 0.001 as determined by unpaired Student’s t test, comparing medLN to any other LN. Data are pooled from two (A–D; n = 8) or three (E; n = 11) independent experiments.$

A substantive subset of influenza-specific memory CD8⁺ T cells isolated from the medLN express residency markers. (A) Mice received Thy1.1 P14 cells 1 d before i.n. infection with PR8-gp33. 85 d later, Thy1.1 expression was measured on P14 cells isolated from medLN by flow cytometry. (B) Ratio of Thy1.1^bright to Thy1.1^dim P14 cells isolated from the indicated tissues (plots were gated on the i.v.^neg fraction of Thy1.1⁺ CD8⁺ T cells). Data displayed on a log2 scale, and numbers above bars represent ratio of Thy1.1^bright to Thy1.1^dim. (C and D) Phenotype of P14 cells described in A. Shaded quadrants highlight T_RM phenotypes. (E) Ratio of Thy1.1⁺ P14 CD8⁺ T cells isolated from medLN versus lung nondraining SLO (40–60 d after i.n. influenza infection). Frequency of medLN P14 cells among total medLN CD8⁺ T cells set at 1. iLN, inguinal LN; cLN, cervical LN; mesLN, mesenteric LN. Scale is log2. (F) Histological comparison of P14 cell abundance and Thy1.1 staining intensity within the medLN and iLN 60 d after influenza infection. Data are represented as mean ± SEM. ***, P < 0.001 as determined by unpaired Student’s t test, comparing medLN to any other LN. Data are pooled from two (A–D; n = 8) or three (E; n = 11) independent experiments.