Figure S2.
Myeloid cells in organotypic hippocampal slices gained from CX3CR1.GFP+/− mice show interactions similar to myeloid cells in organotypic hippocampal slices gained from CX3CR1.GFP+/+. 24-h co-cultures of organotypic hippocampal slices from CX3CR1.GFP+/− pups with pathogenic B6.2D2.CFP.Th17 cells were stained with Image-iT LIVE Red Caspase-3 and -7 Detection Kit to identify apoptotic cells and imaged over a time period of 20 min with two-photon microscopy. The pie chart compares the distribution (%) of interaction modes between myeloid cells and viable 2D2.CFP.Th17 cells (n = 4 organotypic slices from two different experiments), and contingency bars reflect the respective distribution of engulfment, escape, and engulfment attempt. All interactions detectable in organotypic hippocampal slices from CX3CR1.GFP+/− pups (Fig. 4 C) were observed.

Myeloid cells in organotypic hippocampal slices gained from CX3CR1.GFP+/− mice show interactions similar to myeloid cells in organotypic hippocampal slices gained from CX3CR1.GFP +/+. 24-h co-cultures of organotypic hippocampal slices from CX3CR1.GFP+/− pups with pathogenic B6.2D2.CFP.Th17 cells were stained with Image-iT LIVE Red Caspase-3 and -7 Detection Kit to identify apoptotic cells and imaged over a time period of 20 min with two-photon microscopy. The pie chart compares the distribution (%) of interaction modes between myeloid cells and viable 2D2.CFP.Th17 cells (n = 4 organotypic slices from two different experiments), and contingency bars reflect the respective distribution of engulfment, escape, and engulfment attempt. All interactions detectable in organotypic hippocampal slices from CX3CR1.GFP+/− pups (Fig. 4 C) were observed.

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