Detailed classification of interactions between myeloid cells and T cells in organotypic hippocampal slice cultures. 24-h co-cultures of organotypic hippocampal slices from CX3CR1^GFP pups with pathogenic B6.2D2.RFP.Th17 cells were imaged over a time period of 10 min (n = 4 organotypic slices from three different experiments). (A) Characterization of all detectable interactions between myeloid cells (green) and T cells (red) ex vivo. Scale bars = 15 µm. Arrows point to an example of the indicated interaction. (B) Immunohistochemical staining of CX3CR1⁺ myeloid cells (anti-GFP, green), CD4⁺ T cells (anti-CD4, red), and cell nucleus (DAPI, blue). Asterisks mark engulfed T cells. Scale bars = 30 µm. For better visualization, single stainings for two engulfment interactions are shown (right panel).