Figure 1.
Viable T cells in EAE lesions can be engulfed in vivo by CX3CR1GFP cells without subsequent cell death. EAE was induced in CX3CR1GFP (green) mice or CX3CR1.GFPxRag mice via the passive transfer of B6.2D2.RFP.Th17 cells (red). At the peak of disease, ∼11 d after immunization, interactions were imaged in vivo with intravital two-photon imaging (n = 12 mice from three different experiments). (A) Time-lapse surface analysis of a representative full engulfment (minutes 3 and 4) of a red fluorescent Th17 cell by a green myeloid cell, followed by the escape from this engulfment (minutes 5–10). Scale bars = 10 µm. (B–D) Magnified and 3D-rotated surface analysis of the sequence from (B) full engulfment and (C) escape to (D) full T cell detachment. Scale bars = 15 µm. Arrows point to an escaping T cell.

Viable T cells in EAE lesions can be engulfed in vivo by CX3CR1GFP cells without subsequent cell death. EAE was induced in CX3CR1GFP (green) mice or CX3CR1.GFPxRag mice via the passive transfer of B6.2D2.RFP.Th17 cells (red). At the peak of disease, ∼11 d after immunization, interactions were imaged in vivo with intravital two-photon imaging (n = 12 mice from three different experiments). (A) Time-lapse surface analysis of a representative full engulfment (minutes 3 and 4) of a red fluorescent Th17 cell by a green myeloid cell, followed by the escape from this engulfment (minutes 5–10). Scale bars = 10 µm. (B–D) Magnified and 3D-rotated surface analysis of the sequence from (B) full engulfment and (C) escape to (D) full T cell detachment. Scale bars = 15 µm. Arrows point to an escaping T cell.

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