Quantification of TNF-α in IBA1⁺ cells. (A and B) Confocal maximum intensity z-projections of combined RNAscope in situ hybridization and IHC to visualize TNF-α transcript and IBA1 protein, respectively, in WT mouse spinal cord lesions at 4 dpi. The amount of TNF-α in IBA1⁺ cells was determined by the colocalized signal of TNF-α with IBA1 per total amount of TNF-α signal. n = 4 lesions. All data are mean ± SEM (error bars). Scale bar: 20 µm.