Proteome analysis of cultured microglia. (A and B) Volcano plots of regulated proteins in myelin-treated versus vehicle-treated WT microglia and myelin-treated versus vehicle-treated Myd88^−/− microglia, respectively. The negative log₁₀ transformed P value is plotted against the log₂ transformed LFQ intensity ratios for each protein. Proteins with a P value <0.05 are indicated as red circles, whereas proteins with a P value >0.05 are indicated as blue circles. The hyperbolic curves indicate the threshold of a permutation-based FDR correction for multiple hypotheses (FDR: P = 0.05, s0 = 0.1).