Figure S2.
Myelination and phagocyte infiltration are not affected in myd88−/− noninjected larvae. (A) Confocal maximum intensity z-projections of the spinal cord of noninjected larvae over time, showing the myelinated spinal cord (magenta) and phagocytes (cyan) in WT and myd88−/− larvae. (B) Quantification of myelination determined by the total amount of mbp:mCherry-CAAX signal in the dorsal spinal cord. n = 15–19, 11–17, and 13–17at 6 hpi, 2 dpi, and 4 dpi, respectively. (C) Quantification of phagocyte infiltration in the spinal cord determined by the total amount of mpeg1:eGFP signal. n = 15–19, 11–17, and 13–17at 6 hpi, 2 dpi, and 4 dpi, respectively. (D) Confocal maximum intensity z-projections of the spinal cord of noninjected larvae at 4 dpi, showing the myelinated spinal cord (magenta) and the nuclei of mature oligodendrocytes (cyan) in WT and myd88−/− larvae. (E) Quantification of the number of mature oligodendrocytes present in the spinal cord determined by the number of mbp:nls-eGFP–positive nuclei. n = 10 or 8 animals. (F) Confocal maximum intensity z-projections of the spinal cord of noninjected larvae at 4 dpi, showing the myelinated spinal cord (cyan) and the nuclei of oligodendrocyte precursor cells (magenta) in WT and myd88−/− larvae. (G) Quantification of the number of OPCs present in the spinal cord determined by the number of olig1:nls-mApple–positive nuclei. n = 5 or 7 animals. (H and I) Electron micrographs of 6 dpf noninjected dorsal spinal cord cross sections in WT and myd88−/− larvae. Total number of myelinated axons was determined in a representative cross section per animal. n = 3 animals. All data are mean ± SEM (error bars). Scale bars: 50 µm in A; 20 µm in D and F; 1 µm in H.

Myelination and phagocyte infiltration are not affected in myd88−/− noninjected larvae. (A) Confocal maximum intensity z-projections of the spinal cord of noninjected larvae over time, showing the myelinated spinal cord (magenta) and phagocytes (cyan) in WT and myd88−/− larvae. (B) Quantification of myelination determined by the total amount of mbp:mCherry-CAAX signal in the dorsal spinal cord. n = 15–19, 11–17, and 13–17at 6 hpi, 2 dpi, and 4 dpi, respectively. (C) Quantification of phagocyte infiltration in the spinal cord determined by the total amount of mpeg1:eGFP signal. n = 15–19, 11–17, and 13–17at 6 hpi, 2 dpi, and 4 dpi, respectively. (D) Confocal maximum intensity z-projections of the spinal cord of noninjected larvae at 4 dpi, showing the myelinated spinal cord (magenta) and the nuclei of mature oligodendrocytes (cyan) in WT and myd88−/− larvae. (E) Quantification of the number of mature oligodendrocytes present in the spinal cord determined by the number of mbp:nls-eGFP–positive nuclei. n = 10 or 8 animals. (F) Confocal maximum intensity z-projections of the spinal cord of noninjected larvae at 4 dpi, showing the myelinated spinal cord (cyan) and the nuclei of oligodendrocyte precursor cells (magenta) in WT and myd88−/− larvae. (G) Quantification of the number of OPCs present in the spinal cord determined by the number of olig1:nls-mApple–positive nuclei. n = 5 or 7 animals. (H and I) Electron micrographs of 6 dpf noninjected dorsal spinal cord cross sections in WT and myd88−/− larvae. Total number of myelinated axons was determined in a representative cross section per animal. n = 3 animals. All data are mean ± SEM (error bars). Scale bars: 50 µm in A; 20 µm in D and F; 1 µm in H.

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