Delayed phagocyte efflux in myd88^−/− zebrafish larvae with impaired remyelination. (A–C) Confocal maximum intensity z-projections of the spinal cord lesion over time show the myelinated spinal cord (magenta) and phagocytes (cyan) in WT and myd88^−/− larvae injected with LPC. Phagocyte infiltration was determined by the total amount of mpeg1:eGFP signal in the lesion. Myelination was determined by the total amount of mbp:mCherry-CAAX signal in the dorsal spinal cord. n = 15–19, 11–17, and 13–17 animals at 6 hpi, 2 dpi, and 4 dpi, respectively. (D and E) Confocal maximum intensity z-projections of the spinal cord lesion at 4 dpi show the myelinated spinal cord (magenta) and the nuclei of mature oligodendrocytes (cyan). The number of mature oligodendrocytes was determined by counting the number of mbp:nls-eGFP–positive nuclei. n = 7 or 10 animals. (F and G) Confocal maximum intensity z-projections of the spinal cord lesion at 4 dpi show the myelinated spinal cord (cyan) and the nuclei of OPCs (magenta). The number of OPCs was determined by counting the number of olig1:nls-mApple–positive nuclei. n = 7 or 8 animals. Lateral views of the lesion site are shown; anterior is left and dorsal is down. All data are mean ± SEM (error bars). *, P < 0.05; ****, P < 0.0001 by two-way ANOVA test, with Tukey’s multiple comparisons test in B and C and unpaired t test with Welch's correction in E and G. Control noninjected larvae WT and myd88^−/− animals are included in the statistical analysis in B and C and are represented in Fig. S3, A–C. Scale bars: 50 µm in A; 20 µm in D and F.