Characterization of WT and Myd88^−/− zebrafish and mice in noninjected and injected conditions. (A) Confocal maximum intensity z-projections of the spinal cord at 2 and 4 dpi showing lysosomes (magenta) in the phagocytes (cyan) in noninjected WT larvae. (B) Quantification of the amount of lysosomes in phagocytes determined by the colocalized signal of LysoTracker with mpeg1:eGFP relative to the total amount of mpeg1:eGFP signal. n = 6 or 10 animals. (C and D) Luxol Fast Blue staining of mouse spinal cord lesions at 4 dpi. Lesion area was determined by the lack of staining. n = 6 or 7 lesions. (E and F) IBA1⁺ microglia/macrophages were recruited to LPC-induced demyelinated lesions of both WT and Myd88^−/− mice at 4 dpi. n = 6 or 7 lesions. (G and H) At 7 dpi, the proportion of the volume (V) occupied by IBA1⁺ cells in the lesions was similar in Myd88^−/− and WT mice. n = 9 lesions. All data are mean ± SEM (error bars). Scale bars: 20 µm in A; 50 µm in C; 100 µm in E and G.