Characterization of myelin and axonal damage in the zebrafish larvae LPC model. (A–C) Confocal maximum intensity z-projections of the spinal cord show the myelinated spinal cord (cyan) and the axons (magenta) at 2 dpi in noninjected larvae and larvae injected with LPC. n = 16 or 17 animals. (D) Electron micrographs of serial cross sections of the spinal cord lesion at 2 and 4 dpi in WT larvae injected with LPC. (D, a, b, and d–f) Close-up images showing myelin fragments in a demyelinating lesion at 2 dpi. (D, b and c) Close-up images showing a phagocyte (blue) engulfing myelinated axons at 2 dpi. (D, c and e) Close-up images showing lysosomes inside phagocytes (arrow in e) at 2 dpi. (D, b, d, f, and j) Close-up images showing demyelinated axons (asterisks in d and j, at 2 and 4 dpi, respectively). (D, g, h, j, and k) Close-up images showing the presence of partially myelinated axons at 4 dpi (arrowheads in g and k). All data are mean ± SEM (error bars). *, P < 0.05; ****, P < 0.0001 by one-way ANOVA, with Tukey’s multiple comparisons test in B or Kruskal-Wallis test with Dunn’s multiple comparisons test in C. Scale bars: 50 µm in A; 5 µm in D.