Figure 1.
The zebrafish larvae LPC model of de- and remyelination. (A–C) Confocal maximum intensity z-projections of a spinal cord lesion show the myelinated spinal cord (magenta) and phagocytes (cyan) over time. Phagocyte infiltration was determined by the total amount of mpeg1:eGFP signal in the lesion. Myelination was determined by the total amount of mbp:mCherry-CAAX signal in the dorsal spinal cord. n = 9–12, 15–16, 13–20, and 4–8 animals at 6 hpi, 2 dpi, 4 dpi, and 7 dpi, respectively. (D and E) Confocal maximum intensity z-projections of a spinal cord lesion show the myelinated spinal cord (magenta) and the nuclei of mature oligodendrocytes (cyan) over time in noninjected larvae and larvae injected with LPC. The number of mature oligodendrocytes was determined by counting the mbp:nls-eGFP positive nuclei. n = 20–21, 16–21, 20–22, and 12–15 animals at 6 hpi, 2 dpi, 4 dpi, and 7 dpi, respectively. (F and G) Confocal maximum intensity z-projections of the spinal cord lesion show the myelinated spinal cord (cyan) and the nuclei of oligodendrocyte precursor cells (magenta) over time in noninjected larvae and larvae injected with LPC. The number of OPCs was determined by counting the olig1:nls-mApple–positive nuclei. n = 8–10, 12–16, 16, 17, and 16 or 17 animals at 6 hpi, 1 dpi, 2 dpi, 3 dpi, and 4 dpi, respectively. (H and I) Confocal maximum intensity z-projections of the spinal cord lesion at 4 dpi show the myelinated spinal cord (cyan). Myelination was determined by the total amount of mbp:eGFP-CAAX signal in the dorsal spinal cord. n = 8–14 animals. Lateral views of the lesion site are shown; anterior is left and dorsal is down. All data are mean ± SEM (error bars). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by two-way ANOVA test in B, C, E, and G or one-way ANOVA in I, with Tukey’s multiple comparisons test. Scale bars: 50 µm in A and H; 20 µm in D and F.

The zebrafish larvae LPC model of de- and remyelination. (A–C) Confocal maximum intensity z-projections of a spinal cord lesion show the myelinated spinal cord (magenta) and phagocytes (cyan) over time. Phagocyte infiltration was determined by the total amount of mpeg1:eGFP signal in the lesion. Myelination was determined by the total amount of mbp:mCherry-CAAX signal in the dorsal spinal cord. n = 9–12, 15–16, 13–20, and 4–8 animals at 6 hpi, 2 dpi, 4 dpi, and 7 dpi, respectively. (D and E) Confocal maximum intensity z-projections of a spinal cord lesion show the myelinated spinal cord (magenta) and the nuclei of mature oligodendrocytes (cyan) over time in noninjected larvae and larvae injected with LPC. The number of mature oligodendrocytes was determined by counting the mbp:nls-eGFP positive nuclei. n = 20–21, 16–21, 20–22, and 12–15 animals at 6 hpi, 2 dpi, 4 dpi, and 7 dpi, respectively. (F and G) Confocal maximum intensity z-projections of the spinal cord lesion show the myelinated spinal cord (cyan) and the nuclei of oligodendrocyte precursor cells (magenta) over time in noninjected larvae and larvae injected with LPC. The number of OPCs was determined by counting the olig1:nls-mApple–positive nuclei. n = 8–10, 12–16, 16, 17, and 16 or 17 animals at 6 hpi, 1 dpi, 2 dpi, 3 dpi, and 4 dpi, respectively. (H and I) Confocal maximum intensity z-projections of the spinal cord lesion at 4 dpi show the myelinated spinal cord (cyan). Myelination was determined by the total amount of mbp:eGFP-CAAX signal in the dorsal spinal cord. n = 8–14 animals. Lateral views of the lesion site are shown; anterior is left and dorsal is down. All data are mean ± SEM (error bars). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by two-way ANOVA test in B, C, E, and G or one-way ANOVA in I, with Tukey’s multiple comparisons test. Scale bars: 50 µm in A and H; 20 µm in D and F.

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