Kupffer cells are central to the removal of desialylated platelets under homeostatic conditions. Mice were Kupffer cell depleted using clodronate liposomes, and the platelet population was followed over time. (A) Representative flow cytometry plots showing a shift in the total platelet population toward higher galactose expression over time. (B) Quantification of the galactose exposure in the total platelet population over time. Data represent means ± SEM (n = 5 mice/d). MFI, mean fluorescence intensity. (C) Representative flow cytometry plots showing the change in CD41⁺ Thiazole Orange^hi young platelet population in the blood of control mice and mice on day 5 after Kupffer cell depletion. (D) Quantification of the CD41⁺ Thiazole Orange^hi young platelet population in the blood from control mice and mice on day 5 after Kupffer cell depletion. Data represent means ± SEM (n = 5 mice per group). (E) Total platelet count was analyzed using flow cytometry of blood from control mice and mice on day 5 after Kupffer cell depletion. Data represent means ± SEM (n = 5 mice per group). One-way ANOVA with post-hoc testing (B) and unpaired two-tailed t test (D and E) were used to determine statistical significance. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Results from all experiments shown are representative of at least three independent experiments. KC, Kupffer cell.