Recruited MPs carry on myelin degradation and clearance. (a) Time-lapse TPLSM 3D sequence shows ECFP⁺ cell displacement in sciatic nerve 5 d after CCI. Scale bar = 15 µm. (b) Time-lapse TPLSM 3D sequence shows encapsulation of the myelin sheath by ECFP⁺ cell in region of myelin breakdown. Scale bar = 10 µm. (c) Time-lapse TPLSM 3D sequence shows ECFP⁺ phagocytic cell extending long protrusion along myelin sheath (indicated by white arrow). Scale bar = 10 µm. (d) TPLSM image with 3D-masking illustrates the accumulation of ECFP⁺ cells (cyan) in region of highly degraded myelin (yellow) compared with less degraded myelin (purple). Scale bar = 50 µm. Dynamic studies are representatives of four different mice from independent experiments. (e) The density of ECFP⁺ cells in sciatic nerves 10 d after CCI correlates with higher myelin degradation (according to myelin roundness measurements from TPLSM 3D images). Plots represent fields collected from three different nerves processed independently.