MP subsets are functionally distinct. (a) Images of sciatic nerve cryosection 20 d after CCI of MacBlue x Cx3cr1^egfp/+ mice shows the distribution of ECFP⁺ (blue spots) and EGFP⁺ (green spots) relative to the constriction site (white dashed line). Scale bar = 150 µm. (b) Graphs show the relative frequency of ECFP⁺ (left panel) and EGFP⁺ (right panel) cells as a function of the distance from to the constriction at indicated days after injury (n = 3–5 mice per time point processed independently; P values of two-way ANOVA are indicated). (c) Phenotypic characterization gated on of EGFP⁺ and ECFP⁺ MPs (Fig. 1 e) in sciatic nerves 10 d after CCI. Mean fluorescence intensity ± SD are indicated for each subset (n = 6 mice out of two experiments). (d) Hierarchical clustering and Volcano plot of the 124 genes differentially expressed between ECFP⁺ and EGFP⁺ sorted MPs at d14 after CCI (n = 3 independent sorting from a pool of three mice each; fold change [FC] threshold is set to 2, and FDR P value < 0.05).