Different MP subsets accumulate in sequential waves. (a) TPLSM images of sciatic nerve cryosections of MacBlue × Cx3cr1^egfp/+ mice showing the kinetics of fluorescent cell accumulation following CCI. Images represent magnifications of a wider field of tiled and stitched images of the nerve sections at respective days after CCI, Scale bar = 30 µm. (b) Quantification (mean ± 95% CI) of the absolute number per square millimeter (left panel) and relative proportion of EGFP⁺ and ECFP⁺ cells (right panel) from cryosections (n = 4–5 mice per time point processed independently; P value of two-way ANOVA with Bonferroni multiple comparison tests are indicated). (c) 3D TPLSM images showing typical shapes of ECFP⁺ and EGFP⁺ cells in injured nerve. Scale bar = 10 µm. (d) Heatmaps of gene with significant differential expression between ECFP⁺ and EGFP⁺ MPs (fold change >2 with FDR <0.05) and specifically enriched in indicated functional clusters according to IPA (range P values for each functional cluster are indicated). Three independent RNA isolations were prepared from a pool of three mice each. See also Fig. S2. **, P < 0.01; ***, P < 0.001.