MP accumulation during WD. (a) TPLSM images show the specificity of CARS signal from the myelin sheath and the SHG from collagen fibers in the sciatic nerve of a C57Bl6 mouse. Scale bar = 10 µm. (b) Accumulation of CD45⁺ CD11b⁺ cells monitored by flow cytometry in injured nerves, compared with contralateral healthy nerves 10 d after CCI (n = 6 mice out of two experiments; mean percentage ± SD are indicated). (c) kinetic of myeloid cell infiltration following CCI monitored by flow cytometry. Dot plots are gated on CD45⁺ CD11b⁺ cells. Neutrophils are excluded by Ly6G expression, inflammatory Mo’s are defined as Ly6C^high CD64^low, and MPs are defined as Ly6C^low/− CD64⁺. I-A^b acquisition on MPs is followed. Plots with a too-low number of cells are not depicted (n = 6 mice out of two experiments; mean percentage ± SD are indicated).