Macrophage adhesion and macrophage-driven clotting cooperatively promote peritoneal bacterial clearance. (A) Quantification of TAT complex in peritoneal fluid and plasma in the steady state or after E. coli infection. (B) Quantification of TAT complex in peritoneal fluid 4 h after E. coli infection. Ctrl, control. (C) CFUs per microliter peritoneal fluid from untreated, hirudin-treated WT, Lyz2^Cre;Talin^fl/fl mice, hirudin-treated Lyz2^Cre;Talin^fl/fl mice, and clodronate liposome-treated mice 4 h after E. coli infection. (D) CFUs per microliter peritoneal fluid from Gata6^fl/fl and Lyz2^Cre;Gata6^fl/fl mice 4 h after E. coli infection. (E) CFUs per microliter peritoneal fluid from C57BL/6J treated with or without hirudin 4 h after E. coli infection. (F) CFUs per microliter peritoneal fluid from control and F5^−/−;AlbF5Tg mice 4 h after E. coli infection. One-way ANOVA was used to test statistical significance, except for C, D, and F and the F5^−/−;AlbF5Tg groups in B, which were examined by a two-tailed t test. Symbols represent individual mice studied. Error bars represent ± SEM. All experiments were repeated at least two or three times. *, P < 0.05; **, P < 0.01; ***, P < 0.001.