FV and TF from resident peritoneal macrophages are responsible for peritoneal fluid clotting. (A) Quantification of FV activity in plasma or peritoneal fluid of various genotypes of mice or after bone marrow transplant of indicated donor genotypes into irradiated WT recipients (last bars on the right). Zym, zymosan. (B) Quantification of LPMs 3 h after zymosan injection. (C) Quantification of LPMs 3 h after zymosan injection. One-way ANOVA was used to test statistical significance, except for the bone marrow chimera result in B and the hF^+/+ groups and F3^fl/fl groups in C, which used a two-tailed t test. Symbols represent individual mice studied. Error bars represent ± SEM. All experiments were repeated at least two or three times. *, P < 0.05; **, P < 0.01; ***, P < 0.001.