Coagulation and adhesion additively cooperate to account for the MDR in response to inflammation. (A) Gene array analysis (from ImmGen; n = 3 separate pools) of classical coagulation factors in major tissue resident macrophages, including those from the spleen, central nervous system (CNS), lung, and peritoneum. (B) Quantification of LPMs in peritoneal lavage 3 h after zymosan injection i.p. when clotting and/or adhesion was inhibited. (C) Aggregates retrieved from the peritoneum 5 h after zymosan injection. (D–G) Immunofluorescence staining of the aggregates for fibrin(ogen) and macrophage markers. D and G are stained frozen sections of the clots; E and F are whole-mount preparations. Scale bars represent 100 µm (D and G), 50 µm (E), and 10 µm (F). (H) Flow cytometry on peritoneal exudate cells from untreated mice (left), 3 h after zymosan i.p. (middle), and clots 3 h after zymosan i.p. (right). (I) Quantification of LPMs 3 h after zymosan injection in clots and omenta in WT and Lyz2Cre;Tln1fl/fl mice. One-way ANOVA was used to test statistical significance. Symbols represent individual mice studied. Error bars represent ± SEM. All experiments were repeated at least two or three times. **, P < 0.01; ***, P < 0.001.
