Figure 7.
Figure 7. CCR8 on ILC2s is critical for efficient type 2 immunity against N. brasiliensis infection upon adoptive cell transfer. (A–F) Sorted WT or Ccr8−/− ILC2s were adoptively transferred into Rag2−/−il2rg−/− mice or received no ILC2s (control) and infected with N. brasiliensis. (A) Parasite eggs were counted in stool samples. (B) Mice were sacrificed after 10 d, and adult worm counts in small intestinal tissues were determined. (C–E) Ccl1, Gata3, and Il9 expression was determined in lung and small intestinal tissues by qPCR. (F) AB-PAS staining was performed from lung paraffin sections. Scale bars are 50 µm. Pictures show results of one representative animal. Graphs in A–E show pooled data of two independent experiments with two to five mice in each experimental group. Data represent mean ± SEM. *, P ≤ 0.05 by one-way ANOVA.

CCR8 on ILC2s is critical for efficient type 2 immunity against N. brasiliensis infection upon adoptive cell transfer. (A–F) Sorted WT or Ccr8−/− ILC2s were adoptively transferred into Rag2−/−il2rg−/− mice or received no ILC2s (control) and infected with N. brasiliensis. (A) Parasite eggs were counted in stool samples. (B) Mice were sacrificed after 10 d, and adult worm counts in small intestinal tissues were determined. (C–E) Ccl1, Gata3, and Il9 expression was determined in lung and small intestinal tissues by qPCR. (F) AB-PAS staining was performed from lung paraffin sections. Scale bars are 50 µm. Pictures show results of one representative animal. Graphs in A–E show pooled data of two independent experiments with two to five mice in each experimental group. Data represent mean ± SEM. *, P ≤ 0.05 by one-way ANOVA.

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