ILC2-specific deletion of CCR8 results in impaired immunity against N. brasiliensis infection. (A–K) Mixed bone marrow (BM) chimeras with 80% Tie2^creRora^fl/sg and 20% WT (ILC2^WT) or Ccr8^−/− (ILC2^Ccr8−/−) bone marrow were generated. Additionally, chimeras with 100% Tie2^creRora^fl/sg (ILC2^KO) bone marrow were created. After 8 wk, mice were infected with 500 L3 N. brasiliensis larvae or left untreated (control). (B) To confirm the absence of CCR8 on ILC2s in ILC2^Ccr8−/− mice, flow cytometry of lung ILC2s (Lin⁻Thy1⁺KLRG1⁺ST2⁺) and lineage⁺ lymphocytes was performed. CCR8 surface expression was detected using fluorophore-coupled recombinant CCL1 (CCL1-AF647). (C) Parasite egg counts were determined in stool samples. (D) Adult worm counts in small intestinal tissues were determined 12 dpi. (E–J) ILC2 (E; GATA3⁺Thy1⁺Lin⁻), eosinophil (F; CD11b⁺SiglecF⁺SSC^hi), and Th2 (G; GATA3⁺Thy1⁺CD4⁺Lin⁺) numbers per lung were analyzed by flow cytometry 12 dpi. Ccl1 (H), Il9 (I), and Il13 (J) expression in lung tissues was determined by qPCR 12 dpi. (K) AB-PAS staining was performed from lung paraffin sections. Scale bars are 50 µm. Pictures show results of one representative animal and quantification of one representative experiment with three to five animals in one experimental group. Graphs in B–J show pooled data of two representative experiments out of three independent experiments in total. Each dot represents one animal. Data represent mean ± SEM. *, P ≤ 0.05; **, P ≤ 0.01; Mann–Whitney U tests (B) or one-way ANOVA (D–J).